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[Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD]
Shi-Quan Liu1, Xiao-Jun Li, Qing-Bo Yu
1Department of Chemistry and Environment Engineering, Hunan City University, Yiyang 413000, China. lsq@tom.com
Objective:
To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system.
Method:
Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed.
Result:
The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence.
Conclusion:
The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense
