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Updated: Jul 13, 2026

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[Determination of benzopyrene in red clover extract by high performance liquid chromatography with

Fu-Hua Yan¹, Bo Chen, Jian-Guo Zeng

¹Key Laboratory of Biology & Traditional Chinese Medicine Research ( Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica

|July 28, 2007

Abstract

Objective:

To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.

Method:

Analysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70: 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).

Result:

The linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).

Conclusion:

The method was convenient and accurate with good recovery.

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