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The structure of CMS2MS2, a mitogenic protein isolated from Carica candamarcensis
Marco Túlio R Gomes1, Marcelo P Bemquerer, Miriam Tereza P Lopes
1Departamentos de Bioquímica e Imunologia, Farmacologia, Instituto de Ciências Biológicas, Universidade Federal Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Abstract:
In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including the catalytic triad composed of Cys(25), His(159), and Asn(175). A phylogenetic tree analysis demonstrated that CMS2MS2 ranks closer to chymopapain than to papain. The overall predicted three-dimensional structure is similar to proteinases from the papain family. These results suggest that minor structural differences within CMS2MS2 must account for its proliferative action.
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