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Updated: Jul 13, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Improved application of the oscillating method for the isoelectric point determination of protein: potential
Andrés Maldonado1, Francisco Vara, Antonio Sillero
1Departmento de Bioquímica, Facultad de Medicina, Instituto de Investigaciones Biomédicas Alberto Sols (UAM/CSIC), Arzobispo Morcillo 4, 28029 Madrid, Spain.
Abstract:
The oscillating method (OM) for the theoretical determination of the pI values, one by one, of proteins and other macromolecules has been previously published [Sillero and Maldonado, Comput. Biol. Med 36 (2006) 157-166]. An improved application of the method, here named as improved oscillating method (IOM), allows the pI determination of group of proteins. This characteristic may be useful to explore the pI value and electric charge of family of enzymes. As an example the pI values of 1630 enzymes collected in a Swiss-Prot data bank (www.expasy.org), as belonging to the enzymes ligases (EC 6. 2. 1. *) is presented. The method also permits the determination of the pI value of any group of proteins stored in data banks provided that they can be supplied to the program in a FASTA format. Free access to IOM can be reached at http://www.bq.uam.es/otros/pical3.zip.
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