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Related Experiment Video

Updated: Jul 13, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

Speciation in Pyricularia inferred from multilocus phylogenetic analysis.

Kenji Hirata¹, Motoaki Kusaba, Izumi Chuma

¹Graduate School of Agriculture, Kobe University, Nada, Kobe 657-8501, Japan.

Mycological Research

|July 28, 2007

Summary

This study used multilocus phylogenetic analysis to identify seven distinct Pyricularia species. Despite clear species delineation, their evolutionary relationships remain complex, suggesting repeated radiation events.

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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

Area of Science:

Mycology
Plant Pathology
Molecular Phylogenetics

Background:

Accurate species identification and understanding evolutionary relationships in fungal pathogens like Pyricularia are crucial for disease management.
Previous studies have relied on limited genetic markers, potentially leading to incomplete phylogenetic resolution within Pyricularia.

Purpose of the Study:

To resolve the phylogenetic relationships among Pyricularia isolates from diverse host plants using a multilocus approach.
To delineate current species boundaries and investigate the evolutionary history of Pyricularia, including potential instances of species radiation.

Main Methods:

Multilocus phylogenetic analysis was performed on Pyricularia isolates using ribosomal DNA-Internal Transcribed Spacer (rDNA-ITS), actin, beta-tubulin, and calmodulin gene loci.
A combined gene tree was constructed and analyzed for concordance and conflict among loci.
Biological and morphological species criteria were integrated with phylogenetic data for species delineation.

Main Results:

The combined gene tree robustly resolved seven distinct monophyletic groups, each supported by all four analyzed loci, indicating clear species boundaries.
Phylogenetic relationships among these seven species were largely unresolved in both single-gene and combined analyses.
Evidence of gene tree conflict suggested that Pyricularia has undergone multiple, repeated species radiation events throughout its evolution.

Conclusions:

The study successfully delineated seven current Pyricularia species based on combined phylogenetic evidence and traditional species concepts.
The unresolved interspecific relationships and observed gene tree conflicts strongly support a model of recurrent species radiation in Pyricularia.
The findings highlight the complex evolutionary history of Pyricularia, necessitating further investigation into the mechanisms driving its diversification.