Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Imaging the vascular wall using confocal microscopy.

Silvia M Arribas1, Craig J Daly, M Carmen González

  • 1Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain. silvia.arribas@uam.es

The Journal of Physiology
|July 28, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term adverse cardiovascular changes induced by cocoa shell extract administration through lactation in fetal undernourished and control rats.

Experimental physiology·2026
Same author

Influence of oestradiol on exercise induced muscular damage and heart rate variability in a non-trained healthy population.

Experimental physiology·2026
Same author

Influence of nutrition pattern on exercise performance, inflammation and muscle damage biomarkers in a non-athlete healthy young cohort.

Scientific reports·2026
Same author

Relationship between heart rate variability and inflammation induced by physical exercise in a sedentary healthy population.

Frontiers in physiology·2025
Same author

Cocoa Shell Extract Restores Redox Balance in Developmental Hypertension in Male Rats: Roles of Nrf2, SOD2 and p-eNOS.

Pathophysiology : the official journal of the International Society for Pathophysiology·2025
Same author

Cocoa shell extract supplementation in hypertensive rats induces outward hypertrophic remodelling in mesenteric resistance arteries, reducing fibrosis.

Experimental physiology·2025
Same journal

Diving exposure and pulmonary stress.

The Journal of physiology·2026
Same journal

Systems modelling of mitochondrial dynamics in different exercise regimes.

The Journal of physiology·2026
Same journal

Central leptin resistance precedes obesity and drives early endocrine dysfunction.

The Journal of physiology·2026
Same journal

Decoding the molecular memory of obesity using machine learning and microRNA dynamics.

The Journal of physiology·2026
Same journal

Kinematic-calcium loops unravel impaired excitation-contraction coupling in MELAS-affected cardioids.

The Journal of physiology·2026
Same journal

hERG1 channels and potential therapeutics for long QT syndrome.

The Journal of physiology·2026
See all related articles

Confocal microscopy and image analysis offer a 3D view of vascular remodelling, enabling detailed study of blood vessel structure and function. This technique visualizes cellular changes and molecular factors, advancing our understanding of vascular health and disease.

Area of Science:

  • Cardiovascular Biology
  • Biomedical Imaging
  • Cellular Biology

Background:

  • Vascular remodelling involves dynamic changes in blood vessel structure, including cell growth, death, and extracellular matrix alterations.
  • Understanding these complex processes requires advanced imaging techniques that provide detailed structural and functional information.

Purpose of the Study:

  • To highlight the utility of fluorescence confocal microscopy combined with image analysis for studying vascular remodelling.
  • To demonstrate the integration of structural and functional assessments of blood vessels under near-physiological conditions.

Main Methods:

  • Utilizing fluorescence confocal microscopy for serial optical sectioning of arterial walls, avoiding physical sectioning.
  • Employing image analysis software for 3D reconstruction, localization, and quantification of vascular structures.

Related Experiment Videos

  • Combining confocal microscopy with pressure myography for simultaneous functional and structural analysis.
  • Using nuclear dyes and fluorescent kits/antibodies for cell identification, quantification, and detection of dynamic events (apoptosis, proliferation, migration).

    • Main Results:

    • Confocal microscopy provides integrated 3D views of arterial wall components and their interrelationships.
    • The technique allows for quantification of cell characteristics (number, shape, orientation) and visualization of dynamic cellular events.
    • Fluorescence intensity measurements enable quantification of vascular cell factors like nitric oxide and superoxide anion.
    • Simultaneous structural and functional data acquisition is possible using combined confocal microscopy and pressure myography.

    Conclusions:

    • Confocal microscopy and image analysis are powerful tools for investigating vascular wall structure and function.
    • These methods offer significant insights into the dynamic process of vascular remodelling in both physiological and pathological states.
    • The technology facilitates a comprehensive understanding of the cellular and molecular mechanisms underlying vascular remodeling.