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Updated: Jul 13, 2026

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
Published on: March 3, 2023
Cell-free extract systems and the cytoskeleton: preparation of biochemical experiments for transmission electron
Margaret Coughlin1, William M Brieher, Ryoma Ohi
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
Abstract:
Cell-free systems can be used to reconstitute complex actin or microtubule-based phenomena. For example, a number of extracts support actin-dependent propulsion of Listeria monocytogenes, whereas Xenopus laevis extracts support formation of a microtubule-based meiotic spindle. Working in vitro opens these complex processes to biochemical dissection. Here, we describe methods to view these in vitro preparations by thin-section electron microscopy.
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