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Updated: Jul 13, 2026

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
Published on: October 19, 2017
Electron microscopy in situ hybridization: tracking of DNA and RNA sequences at high resolution
1Institute of Cellular Biology and Pathology, Ist Faculty of Medicine, Charles University, Prague, Czech Republic.
Abstract:
Electron microscopy in situ hybridization (EM-ISH) represents a powerful method that enables the localization of specific sequences of nucleic acids at high resolution. We provide here an overview of three different nonisotopic EM-ISH approaches that allow the visualization of nucleic acid sequences in cells. A comparison of various methods with respect to their sensitivity and the structural preservation of the sample is presented, with the aim of helping the reader to choose a convenient hybridization procedure. The post-embedding EM-ISH protocol that currently represents the most widely used technique is described in detail, with a special emphasis on the organization of the cell nucleus.
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