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Updated: Jul 13, 2026

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Published on: May 5, 2012

Simultaneous detection of adenovirus RNA and cellular proteins by fluorescent labeling in situ

Eileen Bridge

    Methods in Molecular Medicine
    |July 28, 2007
    PubMed

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    In-situ Hybridization

    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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    Labeling DNA Probes

    DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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