Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Need for Obtaining Pure Cultures

Need for Obtaining Pure Cultures

Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...

Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Non-Tuberculosis mycobacterium speciation using HPLC under Revised National TB Control Programme (RNTCP) in India.

Journal of applied microbiology·2017

Same author

Computational and spectral studies of 6-phenylazo-3-(p-tolyl)-2H-chromen-2-one.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2014

Same author

Crystal structure of (E)-2-(4-meth-oxy-styr-yl)-2,3-di-hydro-1H-perimidine aceto-nitrile monosolvate.

Acta crystallographica. Section E, Structure reports online·2014

Same author

A fatal case of pure giant cell carcinoma of the lung.

Journal of cancer research and therapeutics·2011

Same author

UV protectants for the biopesticide based on Bacillus sphaericus Neide and their role in protecting the binary toxins from UV radiation.

Journal of invertebrate pathology·2009

Same author

Effect of ultraviolet radiation on spore viability and mosquitocidal activity of an indigenous ISPC-8 Bacillus sphaericus Neide strain.

Acta tropica·2008

Search research articles

Related Experiment Video

Updated: Jul 13, 2026

Isolation of Soil Microorganisms Using iChip Technology

Isolation of Soil Microorganisms Using iChip Technology

Published on: January 10, 2025

Uncultivable bacteria: implications and recent trends towards identification.

S Bhattacharya¹, N Vijayalakshmi, S C Parija

¹Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Pondicherry - 605 006, India.

Indian Journal of Medical Microbiology

|July 28, 2007

Summary

Emerging infectious diseases may stem from uncultivable bacteria, posing a growing threat. Molecular genetic methods are crucial for understanding and identifying these enigmatic pathogens in clinical medicine.

More Related Videos

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Published on: January 12, 2024

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Related Experiment Videos

Last Updated: Jul 13, 2026

Isolation of Soil Microorganisms Using iChip Technology

Isolation of Soil Microorganisms Using iChip Technology

Published on: January 10, 2025

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Published on: January 12, 2024

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Area of Science:

Microbiology
Infectious Diseases
Genetics

Background:

Diseases caused by unculturable bacteria represent a significant, yet poorly understood, category of emerging infectious diseases.
The clinical importance of these pathogens is increasingly recognized, necessitating advanced diagnostic and research approaches.

Purpose of the Study:

To discuss the scientific implications of the evolution of unculturable bacteria.
To review current trends in the identification of these pathogens.
To highlight their relevance in clinical medicine.

Main Methods:

Focus on non-culture-based approaches.
Emphasis on molecular genetic methods for pathogen identification.
Review of recent scientific literature and trends.

Main Results:

Molecular methods are pivotal in studying unculturable bacteria.
Identification techniques are rapidly advancing.
Understanding these pathogens is critical for clinical applications.

Conclusions:

Unculturable bacteria are significant emerging pathogens.
Molecular techniques are essential for their study and clinical relevance.
Further research is needed to fully grasp their impact on human health.