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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: Jul 13, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Toward nanoscale genome sequencing.

Declan Ryan1, Maryam Rahimi, John Lund

  • 1Department of Electrical Engineering, University of Washington Paul Allen Center, AE100R Box 352500 Seattle, WA 98195, USA.

Trends in Biotechnology
|July 31, 2007
PubMed
Summary

Miniaturized devices are advancing DNA sequencing, offering cost reductions through

Area of Science:

  • Genomics and Molecular Biology
  • Nanotechnology
  • Bioengineering

Background:

  • Current DNA sequencing technologies face limitations in cost and scalability.
  • Miniaturization presents a pathway to more accessible genomic information.
  • Nanotechnology enables novel physical methods for nucleotide identification.

Purpose of the Study:

  • To review state-of-the-art miniaturized DNA sequencing technologies.
  • To explore the potential of 'on-chip' devices for cost reduction.
  • To identify challenges in achieving a US$1000-genome sequencing goal.

Main Methods:

  • Review of existing DNA sequencing technologies and their miniaturization.
  • Analysis of nano-scale structures and physical measurement techniques.

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Nanopore DNA Sequencing for Metagenomic Soil Analysis

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Related Experiment Videos

Last Updated: Jul 13, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

  • Assessment of novel approaches for direct nucleotide identification.

    • Main Results:

    • Miniaturization of DNA sequencing offers significant cost-reduction opportunities.
    • 'On-chip' devices leverage nano-scale effects for physical nucleotide identification.
    • Novel physical methods bypass traditional chemical sequencing approaches.

    Conclusions:

    • Miniaturized DNA sequencing is a rapidly evolving field with transformative potential.
    • Achieving a US$1000-genome target requires overcoming specific technological hurdles.
    • Nanotechnology-driven physical methods are key to future sequencing advancements.