Related Experiment Video
Updated: Jan 1, 2026

Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule
Published on: November 25, 2015
Aldosterone-induced modification of osmoregulated ENaC trafficking
Naomi Niisato1, Akiyuki Taruno, Yoshinori Marunaka
1Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Abstract:
Aldosterone and osmotic stress are well known to regulate the epithelial Na(+) channel (ENaC)-mediated Na(+) transport in renal epithelial cells. However, we have no information on how aldosterone and osmotic stress interact on stimulation of ENaC-mediated Na(+) transport in renal epithelium. In the present report, we studied how application of aldosterone (1 microM for 1 day) modifies the action of hypotonic stress on the ENaC-mediated Na(+) transport in renal A6 epithelial cells by measuring the benzamil (a specific inhibitor for ENaC)-sensitive short-circuit current. The present study suggests that: (1) most ENaCs in cells without aldosterone treatment are translocated to Golgi apparatus, (2) major parts of aldosterone-generated ENaCs are located at the endoplasmic reticulum, (3) aldosterone diminishes the endocytosis rate of ENaCs from the apical membrane without any significant changes in the insertion rate of ENaCs into the apical membrane, and (4) application of sucrose after hypotonic stress stimulates the endocytosis of ENaCs, and elongates the functional life time of ENaCs by enhancing recycle of ENaCs into the endoplasmic reticulum in a retrograde manner.
More Related Videos
Related Concept Videos
Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion
Endocrine Signaling
Hormonal Regulation
Reabsorption and Secretion in the Loop of Henle
Renal Tubule and Collecting Duct
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
Introduction to Urinary System
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...

