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Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric
Ji-Seon Jeong1, Ha-Jeong Kwon, Yong-Moon Lee
1Department of Oriental Pharmaceutical Sciences, Kyung Hee University, Dongdaemoon-Gu Hoegi-dong #1, Seoul, South Korea.
Abstract:
We have developed an improved analytical method for the determination of sugar phosphates using sodium carbonate (Na(2)CO(3)) for high-performance anion-exchange chromatography-pulsed amperometric detection. The target analytes were separated completely within 10 min using eluent containing 20 mM NaOH and 35 mM Na(2)CO(3). The limit of detection (S/N=3) and quantitation (S/N=10) for analytes were 10-30 ng/mL and 35-100 ng/mL, respectively. Linear dynamic range was 1-30 microg/mL (r(2)> or =0.9998). The RSDs for intra- and inter-day assays were found to be of satisfactory results (0.23-3.09%), and the recoveries from blood spots were 97.62-99.69%.
