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Related Experiment Video

Updated: Jul 13, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
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Published on: May 21, 2021

A titer plate-based polymer microfluidic platform for high throughput nucleic acid purification.

D S-W Park1, M L Hupert, M A Witek

  • 1Center for Bio-Modular Multi-Scale Systems, Louisiana State University, Baton Rouge, LA 70803, USA.

Biomedical Microdevices
|July 31, 2007
PubMed
Summary

This study presents a novel 96-well microfluidic reactor for solid-phase reversible immobilization (SPRI) DNA purification. The developed platform enables rapid and efficient isolation of genomic DNA from bacterial samples, verified by PCR.

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Published on: May 23, 2018

Area of Science:

  • Microfluidics
  • Biotechnology
  • Materials Science

Background:

  • Microfluidic platforms offer advantages for biological sample processing.
  • Solid-phase reversible immobilization (SPRI) is a key technique for nucleic acid purification.
  • Developing integrated, high-throughput SPRI reactors is crucial for molecular diagnostics.

Purpose of the Study:

  • To design and fabricate a 96-well SPRI reactor plate for microfluidic applications.
  • To demonstrate the utility of the SPRI reactor for rapid genomic DNA purification.
  • To validate the performance of the fabricated reactors for bacterial DNA isolation.

Main Methods:

  • Fabrication of nickel mold inserts using UV-LIGA and SU-8 lithography.
  • Replication of SPRI reactors via hot embossing of polycarbonate (PC).
  • Thermal fusion bonding of PC chips and verification of leak-free seals.

Main Results:

  • High-precision nickel mold inserts with vertical sidewalls were successfully fabricated.
  • Polycarbonate SPRI reactors with excellent feature replication fidelity were produced.
  • Leak-free SPRI reactor plates were assembled and demonstrated effective genomic DNA purification from bacterial lysates.

Conclusions:

  • The developed 96-well SPRI reactor plate is a functional microfluidic platform for DNA purification.
  • The fabrication method using UV-LIGA and hot embossing is suitable for producing high-quality SPRI reactors.
  • This technology enables simple, fast, and efficient genomic DNA isolation for downstream applications like PCR.