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Updated: Jul 13, 2026

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
Published on: November 3, 2019
Characterization of bovine patellar cartilage by NMR
Wen Ling1, Ravinder R Regatte, Mark E Schweitzer
1Chemistry Department, New York University, New York, NY 10003, USA.
Abstract:
Metabolic and structural changes in cartilage tissue are thought to be at the root of degenerative joint disease. We identify here the NMR resonances in bovine patellar cartilage tissue by static and high-resolution magic angle spinning (HRMAS) NMR spectroscopy, (1)H-(13)C heteronuclear single-quantum correlation (HSQC) spectroscopy, total correlation spectroscopy (TOCSY), and saturation transfer experiments. Some differences between the patellar cartilage samples studied here and earlier nasal cartilage and intervertebrate disc studies were found. In addition, we show assignments downfield of the water signal, which also includes the assignment of amide and hydroxy protons on the basis of their exchangeability with water. These results will allow an identification of spectroscopic markers of cartilage degradation using techniques such as chemical exchange saturation transfer imaging.
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