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Multiphoton microscopy for monitoring intratissue femtosecond laser surgery effects
Bao-Gui Wang1, Iris Riemann, Harald Schubert
1Lasermicroscopy Research Unit, Institute of Microscopic Anatomy (Anatomy II), Friedrich-Schiller University, Teichgraben 7, 07743 Jena, Germany.
Background And Objective:
Multiphoton microscopy/tomography has been used as a novel diagnostic method for corneal imaging with subcellular resolution. Here, we used this technique to monitor femtosecond laser intratissue surgery effects.
Materials And Methods:
Multiphoton microscopy/tomography on rabbits based on intense 90 MHz femtosecond Ti: sapphire laser was realized at intensities of MW-GW/cm(2), whereas the surgical procedures were performed with the same system at a higher light intensity on the order of TW/cm(2).
Results:
Multiphoton microscopy/tomography proved capable of determining the target of interest, and of visualizing and optically evaluating the in vivo intrastromal laser surgical outcomes with high efficiency. More interesting, using this technique, activated intrastromal keratocytes (myofibroblasts) were detected in vivo 24 hours after intrastromal surgery.
Conclusion:
Multiphoton microscopy/tomography is an efficient and convenient non-invasive imaging method which can be used not only to assess intrastromal microsurgical performance but also to perform in vivo follow-up observations on wound repair.
Insights
Multiphoton microscopy/tomography non-invasively monitors femtosecond laser corneal surgery in rabbits. This advanced imaging technique visualizes surgical outcomes and tracks wound healing, including activated keratocytes, in vivo.
Area of Science:
- Ophthalmology
- Biomedical Imaging
- Laser Surgery
Background:
- Multiphoton microscopy/tomography offers subcellular resolution for corneal imaging.
- Femtosecond laser technology is advancing intratissue surgical procedures.
Purpose of the Study:
- To utilize multiphoton microscopy/tomography for monitoring femtosecond laser intratissue surgery effects.
- To evaluate the efficacy of this imaging technique in assessing surgical outcomes and wound repair.
Main Methods:
- Intense 90 MHz femtosecond Ti:sapphire laser multiphoton microscopy/tomography was applied to rabbits.
- Surgical procedures were conducted using the same laser system at higher intensities (TW/cm²).
Main Results:
- Multiphoton microscopy/tomography effectively identified surgical targets and visualized in vivo intrastromal laser surgery outcomes.
- Activated intrastromal keratocytes (myofibroblasts) were detected 24 hours post-surgery using this method.
Conclusions:
- Multiphoton microscopy/tomography is an efficient, non-invasive tool for assessing microsurgical performance.
- The technique enables in vivo follow-up observations of corneal wound repair post-surgery.
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