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Updated: Jul 13, 2026

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells.

Tri Giang Phan¹, Irina Grigorova, Takaharu Okada

¹Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, California 94143, USA.

Nature Immunology

|July 31, 2007

Summary

Macrophages in lymph nodes rapidly deliver immune complexes to B cells, facilitating antigen capture and immune responses. This highlights a key pathway for B cell-antigen interaction in lymphoid tissues.

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Isolation of Murine Lymph Node Stromal Cells

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Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

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Published on: August 9, 2019

Area of Science:

Immunology
Cell Biology
Lymphoid Tissue Dynamics

Background:

The precise mechanisms governing B cell interactions with antigens within lymphoid tissues remain incompletely elucidated.
The transport pathways for immune complexes to follicular dendritic cells are not well understood.

Purpose of the Study:

To investigate the initial events of B cell-antigen encounter in lymph nodes.
To clarify the role of macrophages and immune complex transport in initiating adaptive immunity.

Main Methods:

Real-time two-photon microscopy was employed to visualize dynamic cellular interactions in lymph nodes.
The study focused on the movement and capture of immune complexes by B cells and macrophages.

Main Results:

Immune complexes were rapidly transported via lymph to subcapsular sinus macrophages.
B cells captured immune complexes from macrophage extensions in a complement receptor-dependent manner.
Cognate B cells acquired antigen-containing immune complexes and migrated towards the T zone.

Conclusions:

Subcapsular sinus macrophages are critical sites for B cell engagement with immune complexes.
Intrafollicular B cell migration is essential for immune complex transport and cognate antigen recognition, advancing immune surveillance.