Related Experiment Video
Updated: Jul 13, 2026
Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
Published on: August 25, 2020
[Study of MSCs in vitro cultured on demineralized bone matrix of mongrel]
Li-qiang Chen1, Ning-yi Li, Rong-Tao Yuan
1Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province,China. clqiang1010@163.com
Purpose:
To manufacture demineralized bone matrix(DBM) of mongrel and to explore the feasibility of DBM as scaffold of bone tissue engineering.
Methods:
Thigh bones of mongrel were degreased, demineralized, deproteined, freezed, dried and sterilized to form DBM. Mesenchymal stem cells(MSCs) were seeded onto the scaffold and their growth were examined by inverted phase contrast microscope and scanning electron microscope.
Results:
DBM had a three-dimensional mesh structure.The mean pore diameter of DBM was (254.39+/-88.71)microm and the pore rate was about 70%.MSCs could adhere to the surface and inner walls of DBM, proliferated well and secreted a large amount of extracellular matrix.
Conclusion:
DBM has satisfactory biocompatibility.
