Rapid determination of hydrogen peroxide using peroxidase immobilized on Amberlite IRA-743 and minerals in honey

Hydrogen peroxide and trace metals (K+, Ca2+, Na+, Mg2+, Mn2+, and Li+) were determined in 14 samples of Brazilian commercial honeys. The method for the determination of H2O2 is based on selective oxidation of H2O2 using an on-line tubular reactor containing peroxidase immobilized on Amberlite IRA-743 resin. Reactors presented high stability for at least 2 weeks under intense use. The results show a simple, accurate, selective, and readily applied method to the determination of H2O2 in honey. The trace metals were determined by capillary zone electrophoresis. Mean contents of 656, 69.1, 71.8, 36.0, 21.4, and 1.70 mg/kg were found, respectively, for K+, Ca2+, Na+, Mg2+, Mn2+, and Li+ in the analyzed honeys. The cations were identified by comparison of the relative migration times of their peaks with the Ba2+ migration time used as reference. The electrophoretic analysis was simple and rapid and did not require any other preparation of sample than dilution and filtration.