Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Evolution of Microbial Genome01:08

Evolution of Microbial Genome

Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stent retriever-assisted intra-arterial lysis for distal vessel occlusions: a feasible pharmacomechanical rescue strategy.

Journal of neurointerventional surgery·2026
Same author

CpG-ODN/Coa-ASC16 nanostructured adjuvant platform as a safe and effective alternative in Bothrops diporus antivenom production.

Archives of toxicology·2026
Same author

Meckel's Diverticulum as a Rare Etiology of Small-Bowel Obstruction in an Otherwise Healthy Adult: A Case Report.

Cureus·2026
Same author

Endovascular Treatment for Medium and Distal Vessel Occlusion Stroke: Are We Missing the Point?

Stroke (Hoboken, N.J.)·2026
Same author

Refining thrombectomy benefit in medium vessel occlusion (MeVO) strokes using imaging and clinical enrichment.

Journal of neurointerventional surgery·2026
Same author

Balloon-Expandable Stenting as a Bridging Therapy in Patients With Acute Stroke and Tandem Occlusions.

Stroke (Hoboken, N.J.)·2026

Related Experiment Video

Updated: Jul 13, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Genome content determination in methicillin-resistant Staphylococcus aureus.

Patrice Francois1, David Hernandez, Jacques Schrenzel

  • 1Service of Infectious Diseases, Genomic Research Laboratory, Department of Internal Medicine, Geneva 14, Switzerland. patrice.francois@genomic.ch

Future Microbiology
|July 31, 2007
PubMed
Summary

Methicillin-resistant Staphylococcus aureus (MRSA) causes significant healthcare costs and hospital stays. Whole-genome characterization using sequencing and microarrays aids in understanding MRSA epidemiology and virulence.

More Related Videos

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Related Experiment Videos

Last Updated: Jul 13, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Staphylococcus aureus is a significant pathogen causing both hospital-acquired and community-acquired infections.
  • Methicillin-resistant Staphylococcus aureus (MRSA) emerged as a global nosocomial issue in the 1980s, increasing costs and hospital stay duration.
  • MRSA infections are associated with a threefold increase in attributable cost and excess hospital stay compared to methicillin-susceptible S. aureus.

Purpose of the Study:

  • To explore the utility of high-throughput sequencing and microarray technologies for characterizing MRSA.
  • To understand the relationship between MRSA genome content, virulence, and epidemicity.
  • To facilitate the development of molecular diagnostic and genotyping assays for MRSA.

Main Methods:

  • Whole-genome characterization using microarrays.
  • Whole-genome characterization using high-throughput sequencing devices.
  • Analysis of genetic-encoded resistance markers and toxin content.

Main Results:

  • High-throughput sequencing has enabled the development of tools for large-scale study of MRSA clinical isolates.
  • Genomic characterization provides data for developing genotyping assays.
  • Genomic data allows for the evaluation of strain virulence by assessing resistance markers and toxin content.

Conclusions:

  • Whole-genome characterization is crucial for understanding MRSA epidemiology and virulence.
  • Sequence information is essential for developing rapid molecular tests for MRSA identification, genotyping, and characterization.
  • These genomic tools are vital for combating the spread and impact of MRSA infections.