Related Experiment Video
Updated: Jul 13, 2026
08:26
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
Biomarker discovery in infectious diseases using SELDI
Andrea Hodgetts1, Michael Levin, J Simon Kroll
1Imperial College London, Department of Paediatrics, Division of Medicine, St Mary's Campus, London, UK. a.hodgetts@imperial.ac.uk
Future Microbiology
|July 31, 2007
Summary
Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) mass spectrometry is a powerful tool for identifying protein biomarkers in infectious diseases. This high-throughput method aids in diagnosing infections and understanding disease progression.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Infectious Diseases
Background:
- Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) is a mass spectrometry technique.
- It requires minimal sample volume and is suitable for high-throughput screening.
- SELDI-TOF has been applied to identify protein signatures and biomarkers for various infectious agents.
Purpose of the Study:
- To highlight the utility of SELDI-TOF mass spectrometry in infectious disease research.
- To demonstrate its application in discovering protein biomarkers.
- To emphasize its role in diagnostics and understanding disease processes.
Main Methods:
- Utilizing SELDI-TOF mass spectrometry for protein analysis.
- Analyzing serum and tissue samples.
- High-throughput screening of protein signatures.
Main Results:
- Successful identification of protein signatures and biomarkers for viral, parasitic, and bacterial infections.
- Demonstrated potential for diagnosing infections like hepatitis, HIV, tuberculosis, and more.
- Established protein biomarkers for predicting disease states and informing on disease mechanisms.
Conclusions:
- SELDI-TOF is a valuable method for infectious disease biomarker discovery.
- Accurate experimental design, sample handling, and controls are critical for successful SELDI-TOF applications.
- Protein signatures identified can significantly aid in clinical diagnostics and disease management.
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...