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Updated: Feb 6, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Published on: March 17, 2023
Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression
Markus Gorfer1, Sylvia Klaubauf, Dragana Bandian
1Fungal Genomics Unit, Austrian Research Centres and BOKU Vienna, Muthgasse 18, 1190 Vienna, Austria. markus.gorfer@boku.ac.at
Abstract:
Hygromycin B resistance was transferred to the sterile mycelia of Cadophora finlandia and Phialocephala fortinii by co-cultivation with Agrobacterium tumefaciens. Constitutively expressed green fluorescent protein (GFP) was also introduced using the same vector. Confocal laser scanning microscopy (CLSM) revealed strong fluorescence of transformants. Both traits were mitotically stable during one year of subculturing on non-selective growth medium. Southern blot analysis showed that the majority of the transformants contained single-copy integrations at random sites in the genome.
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