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Related Experiment Videos

Membrane permeable esterase-activated fluorescent imaging probe.

Youngmi Kim1, Yongdoo Choi, Ralph Weissleder

  • 1Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

Bioorganic & Medicinal Chemistry Letters
|August 1, 2007
PubMed
Summary
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A novel esterase-triggered probe enables bright fluorescent cell labeling. This cyanine-based probe is activated by cellular esterases for enhanced intracellular imaging.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Fluorescence Microscopy

Background:

  • Cyanine dyes are widely used in biological imaging.
  • Cellular esterases play crucial roles in various biological processes.
  • Developing specific probes for cell labeling is essential for biological research.

Purpose of the Study:

  • To develop a novel esterase-triggered probe for cell labeling.
  • To utilize a cyanine-based pH sensitive dye for enhanced fluorescence.
  • To achieve bright intracellular fluorescent signals for cellular imaging.

Main Methods:

  • Synthesis of probe 2 derived from a cyanine-based pH sensitive dye.
  • Investigation of probe permeation into cells.
  • Assessment of probe activation by cellular esterases.

Related Experiment Videos

  • Evaluation of intracellular fluorescence intensity.

    • Main Results:

    • Probe 2 successfully permeated into cells.
    • Hydrolytic activation by cellular esterases was confirmed.
    • A bright fluorescent intracellular signal was observed.
    • The probe demonstrated effective cell labeling capabilities.

    Conclusions:

    • The developed esterase-triggered probe is effective for cell labeling.
    • The probe offers a sensitive method for detecting esterase activity within cells.
    • This probe facilitates enhanced intracellular imaging in biological studies.