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Methods for imaging cell fates in hematopoiesis.

Jennifer Duda1, Mobin Karimi, Robert S Negrin

  • 1Program in Molecular Imaging at Stanford, Stanford University School of Medicine, Stanford, CA, USA.

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Advanced in vivo imaging techniques enable researchers to track cells in living subjects, significantly advancing stem cell research and understanding cell trafficking for injury repair and hematopoietic engraftment in mouse models.

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Area of Science:

  • Biomedical Imaging
  • Stem Cell Biology
  • Hematopoiesis Research

Background:

  • In vivo cell monitoring technologies have revolutionized biological research.
  • Hematopoiesis and stem cell research benefit from advanced imaging for studying cell trafficking, injury repair, and hematopoietic engraftment.

Purpose of the Study:

  • To provide an overview of in vivo imaging technologies and labeling strategies for hematopoietic stem cells in mouse models.
  • To guide researchers in designing and implementing experimental systems for in vivo cell imaging.

Main Methods:

  • Discusses multiple imaging modalities including magnetic resonance imaging (MRI), positron emission tomography (PET), bioluminescence imaging (BLI), and fluorescence imaging.
  • Details various cell labeling techniques required for each imaging modality.
  • Provides protocols for gene delivery into hematopoietic cells for applying these labels.

Main Results:

  • Highlights the advantages and disadvantages of different imaging modalities for specific applications.
  • Emphasizes the necessity of distinct cell labels for each imaging technology.
  • Presents a comprehensive guide to selecting and applying labels for in vivo cell tracking.

Conclusions:

  • In vivo imaging offers powerful tools for studying cell dynamics in hematopoiesis and stem cell transplantation.
  • Proper selection of imaging modality, cell labels, and delivery methods is crucial for successful experimental design.
  • This chapter equips researchers with the knowledge to establish robust in vivo imaging systems for mouse models.