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erg, an ets-related gene, codes for sequence-specific transcriptional activators
1Fels Institute for Cancer Research and Molecular Biology, Temple University, Philadelphia, Pennsylvania 19140.
Oncogene
|December 1, 1991
Summary
The ets-related gene (erg) produces two proteins, erg-1 and erg-2, which bind DNA specifically. These erg proteins function as transcriptional activators, similar to other ets oncogene family members.
Area of Science:
- Molecular Biology
- Oncology
- Virology
Background:
- Avian acute leukemia virus E26 encodes v-myb and v-ets oncogenes.
- The ets oncogene family includes several related genes, such as erg, c-ets-1, and PU.1.
- The erg gene produces at least two protein isoforms, erg-1 and erg-2, via alternative splicing and initiation codon usage.
Purpose of the Study:
- To investigate the DNA-binding properties and transcriptional activity of erg-1 and erg-2 proteins.
- To compare the DNA-binding specificity of erg proteins with other ets family members.
Main Methods:
- Expression of erg-1 and erg-2 proteins in Escherichia coli.
- DNA-binding assays using recombinant erg proteins and specific oligonucleotides.
- Reporter gene assays to assess transcriptional activation by erg proteins.
Main Results:
- Recombinant erg-1 and erg-2 proteins bind DNA in a sequence-specific manner.
- Erg proteins display distinct DNA-binding specificities compared to c-ets-1, ets-2, PU.1/Spi-1, and elk-1.
- The erg gene transactivates reporter genes linked to erg target sequences.
Conclusions:
- Erg-1 and erg-2 are sequence-specific transcriptional activators.
- Erg proteins represent a distinct class within the ets oncogene superfamily.
- The findings contribute to understanding the functional diversity of the ets oncogene family.
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