Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cardiologists' perspectives on sociocultural and structural factors shaping cardiovascular genetic testing.

medRxiv : the preprint server for health sciences·2026

Same author

COMPREHENSIVE GENETIC INVESTIGATION REVEALS HETEROGENEOUS PATHWAYS TO OBSTRUCTIVE SLEEP APNEA.

medRxiv : the preprint server for health sciences·2026

Same author

Prevention and treatment of hypertriglyceridemia-mediated acute pancreatitis: A narrative review.

European journal of internal medicine·2025

Same author

Assessing the views and opinions of psychiatric patients receiving genome-guided treatment within the scope of the PREPARE preemptive pharmacogenomics clinical study.

Frontiers in pharmacology·2025

Same author

Genomic medicine year in review: 2025.

American journal of human genetics·2025

Same author

Leveraging genomic biobanks to enhance genetic testing outcomes for kidney disease.

medRxiv : the preprint server for health sciences·2025

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 6, 2014

Rapid ACCE: experience with a rapid and structured approach for evaluating gene-based testing.

James M Gudgeon¹, Monica R McClain, Glenn E Palomaki

¹Clinical Genetics Institute, Intermountain Healthcare, Salt Lake City, Utah 84103, USA. Jim.Gudgeon@intermountainmail.org

Genetics in Medicine : Official Journal of the American College of Medical Genetics

|August 2, 2007

Summary

The rapid-ACCE model enables quick systematic reviews for emerging genetic tests, summarizing evidence and identifying research gaps efficiently. This approach proves effective for evaluating new genetic technologies within tight timelines and budgets.

More Related Videos

Rapid Characterization of Genetic Parts with Cell-Free Systems

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Related Experiment Videos

Last Updated: Jul 10, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 6, 2014

Rapid Characterization of Genetic Parts with Cell-Free Systems

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Area of Science:

Health Services Research
Genetics
Evidence-Based Medicine

Background:

Emerging genetic tests require timely evaluation to inform clinical practice and policy.
Existing systematic review methods can be time-consuming, delaying the assessment of new technologies.
The ACCE (Assessing the Clinical utility of Genetic Tests) framework provides a structure for evaluating genetic tests.

Purpose of the Study:

To introduce the rapid-ACCE model for evaluating emerging genetic tests.
To report on the initial experience using the ACCE framework for rapid systematic reviews.
To assess the feasibility of timely evidence synthesis for novel genetic diagnostic tools.

Main Methods:

Employed the 44-question ACCE model for systematic review conduct.
Integrated published literature with unpublished data for performance estimation.
Utilized expert input for qualitative aspects and identified knowledge gaps.
Incorporated feedback from outside reviewers into final reports.

Main Results:

Two rapid-ACCE reviews were completed within 6 months each, averaging 100 hours of analyst time.
Reviews were conducted within modest budgets, defining the current evidence base for genetic tests.
Identified knowledge gaps are expected to guide future research agendas.
Collaborating experts and sponsors found value in the rapid review process and outcomes.

Conclusions:

Rapid systematic reviews using the ACCE structure are feasible for evaluating certain emerging genetic tests.
This model effectively summarizes available evidence and highlights critical areas for future research.
The rapid-ACCE approach facilitates timely evidence synthesis for rapidly evolving genetic technologies.