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Updated: Jul 13, 2026

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
Published on: July 7, 2017
Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges
Ting Lei1, Sandrine Jacob, Imen Ajil-Zaraa
1Laboratory of Stem Cell Research, Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland.
Abstract:
Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.
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