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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jul 13, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

An easy method to discover cell membrane antigen with atomic force microscopy.

Wang Shu1, Yang Ting Shu, Han Bao Shi

  • 1Laboratory of Cardiovascular Medical Department, The General Hospital of Chinese People of Liberation Army, Fu Xin Road 28, Beijing, 100853, China. wangshu2002@163.com

Molecular Biology Reports
|August 2, 2007
PubMed
Summary

Atomic force microscopy (AFM) can detect cell surface changes. Researchers found AFM imaging revealed significant differences in mouse mesenchymal stem cell shape and membrane roughness when specific antibodies (CD34, CD44, CD29) were added, indicating potential for antigen analysis.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) provides high-resolution, nanoscale imaging capabilities.
  • Mouse adipose tissue-derived mesenchymal stem cells (ADMSCs) are a relevant model for studying cell surface properties.

Purpose of the Study:

  • To investigate the utility of AFM in detecting changes in ADMSC morphology and membrane roughness upon antibody binding.
  • To determine if AFM can qualitatively assess the presence of cell membrane antigens.

Main Methods:

  • AFM imaging was performed on ADMSCs.
  • Antibodies targeting specific cell membrane antigens (CD34, CD44, CD29, CD45, CD144) were added.
  • Cell shape and average membrane roughness were analyzed before and after antibody addition.

Main Results:

  • Significant changes in ADMSC cell shape and average membrane roughness were observed after adding CD34, CD44, or CD29 antibodies.
  • No significant changes in cell shape or membrane roughness were detected after adding CD45 or CD144 antibodies.
  • AFM successfully differentiated between the effects of different antibodies on cell surface characteristics.

Conclusions:

  • AFM is a valuable tool for analyzing qualitative changes in cell membrane antigen presence.
  • Changes in cell shape and membrane roughness detected by AFM can serve as indicators for specific cell surface antigens.
  • This method offers a potential approach for the qualitative analysis of cell membrane antigens using AFM.