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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
[Identification of human papilloma viruses (HPV) in inflammatory states and ear neoplasms]
Bogdan Rydzewski1, Anna Goździcka-Józefiak, Jerzy Sokalski
1Oddział Otolaryngologiczny Szpitala im. F. Raszei w Poznaniu.
Introduction:
Human Papilloma Virus has a strong relation to oropharyngeal mucosa and is considered to be responsible for a wide range of upper respiratory tract pathologies, like laryngeal papilloma. There's a hypothesis, that it plays a significant role in middle ear chronic inflammations and neoplasm's. MATERIAL AND METHODIC. The examination was carried on a group of 53 patients, 39 of which was suffering from granulation tissue chronic otitis media, 7-cholesteatomatous otitis media, 6--middle ear malignant neoplasm, and 1 middle and/or external ear benign neoplasm. The control group consisted of 5 patients operated on: otosclerosis--4 cases and post-traumatic tympanic membrane perforation--1 case. The material was postoperative tissue, like polyps, inflammatory granulation tissue, cholesteatoma masses and malignant neoplasm's tissue.
Results:
In the whole group of 53 examined cases, HPV DNA was confirmed in 22 cases (41.5%), in that group oncogenic types 16 or 18 in 12 cases (22.6%), and in 14 cases (26.4%) types 6 or 11. In a group of chronic granulomatous otitis media DNA characteristic for Papilloma was identified in 12 cases (25.6%), in it in 9 cases DNA HPV type 6 or 11 was confirmed, and in 7 cases type 16 or 18. Among cholesteatomatous chronic otitis media HPV DNA types 6 or 11 was identified in 70%. In every case of middle ear malignant neoplasm a presence of high-risk DNA Papilloma types 16 or 18 was confirmed. In any case of control group HPV DNA was detected.
Conclusions:
The results has been compared with other authors examinations and it is claimed that they confirm the observation, that Human Papilloma Viruses may be a factor, that might play an important role in pathology of chronic otitis media and ear neoplasm's. It is concluded, that differences in percentages of HPV presence in chronic inflammations (70%) and ear neoplasm's may be explained by viral co-infection during bacterial c. o. m. Viral infection probably evolves carcinogenesis, which leads to a neoplastic growth.
Insights
Human Papilloma Virus (HPV) DNA was detected in 41.5% of middle ear pathologies, including chronic otitis media and neoplasms. High-risk HPV types were prevalent in malignant ear neoplasms, suggesting a role in ear disease development.
Area of Science:
- Otolaryngology
- Virology
- Oncology
Context:
- Human Papilloma Virus (HPV) is linked to oropharyngeal cancers and upper respiratory tract pathologies.
- A hypothesis suggests HPV's role in chronic middle ear inflammations and neoplasms.
- This study investigated HPV presence in various middle ear pathologies.
Purpose:
- To determine the prevalence of Human Papilloma Virus (HPV) DNA in middle ear pathologies.
- To identify specific HPV types associated with chronic otitis media and middle ear neoplasms.
- To explore the potential role of HPV in the pathogenesis of these conditions.
Summary:
- HPV DNA was detected in 41.5% of 53 patients with middle ear conditions.
- Oncogenic HPV types 16/18 were found in 22.6%, and low-risk types 6/11 in 26.4%.
- HPV was present in 70% of cholesteatoma cases and 100% of malignant neoplasms, with high-risk types predominating.
Impact:
- Findings support the hypothesis that HPV may be a significant factor in chronic otitis media and middle ear neoplasms.
- The high prevalence of HPV in malignant neoplasms suggests a role in carcinogenesis.
- Results warrant further investigation into viral co-infections and their role in ear pathology evolution.
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