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Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Published on: September 11, 2020
Construction and validation of a neutrally-marked strain of Pseudomonas fluorescens SBW25
Xue-Xian Zhang1, Paul B Rainey
1NZ Institute of Advanced Study and Institute of Molecular Biosciences, Massey University, Private Bag 102 904, North Shore Mail Centre, Auckland, New Zealand. xx.zhang@auckland.ac.nz
Abstract:
Neutrally marked bacterial strains are useful in many experimental evolution and molecular ecology studies to assess the relative fitness of a given strain. Here we describe the construction and validation of a neutral marker for the model organism Pseudomonas fluorescens SBW25. The marked strain, called SBW25-lacZ, was created by integrating a promoterless 'lacZ into the defective prophage locus of the SBW25 chromosome. Fitness assays conducted in various laboratory media and in planta revealed that the fitness levels of SBW25-lacZ were comparable with the wild-type ancestor.
