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Updated: Jul 13, 2026

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
Early drought-induced changes to the needle proteome of Norway spruce
Constanze Blödner1, Andrzej Majcherczyk, Ursula Kües
1Forstbotanik und Baumphysiologie, Institut für Forstbotanik, Georg-August Universität Göttingen, Büsgenweg 2, 37077 Göttingen, Germany.
Abstract:
To elucidate early drought responses in needles of Norway spruce (Picea abies (Karst.) L.), we subjected 1-year-old seedlings to gradual desiccation for 6 weeks. Four weeks of drought treatment caused a small but significant decrease in photosystem II quantum yield of light-adapted needles (phi(a)) compared with that of well-watered controls. Six weeks of drought treatment reduced phi(a) and the photosystem II quantum yield of dark-adapted needles (phi) by 50 and 8%, respectively, and reduced shoot water potential by 0.7 MPa, but had no measurable effect on needle relative water content. After two weeks of drought treatment, and before there was a discernible effect of drought on phi or a statistically significant effect on shoot water potential, needles were analyzed for changes in protein composition. Five out of several hundred detected proteins in needles of drought-treated plants showed consistent changes compared with control leaves. The proteins were identified by LC-MS/MS as components of the oxygen-evolving complex (oxygen evolving enhancer protein 2), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, and one protein of unknown function, whose mRNA was found in a previous screen of wound- and methyl-jasmonate-induced bark proteins.
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