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Published on: June 12, 2018
Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system
Motoaki Wakiyama1, Koji Takimoto, Osamu Ohara
1RIKEN Genomic Sciences Center, Protein Research Group, Tsurumi, Yokohama 230-0045, Japan.
Abstract:
MicroRNAs (miRNAs) are incorporated into miRNP complexes and regulate protein expression post-transcriptionally through binding to 3'-untranslated regions of target mRNAs. Here we describe a recapitulation of let-7 miRNA-mediated translational repression in a cell-free system, which was established with extracts prepared from HEK293F cells overexpressing miRNA pathway components. In this system, both the cap and poly(A) tail are required for the translational repression, and let-7 directs the deadenylation of target mRNAs. Our work suggests that let-7 miRNPs containing Argonaute and GW182 impair the synergistic enhancement of translation by the 5'-cap and 3'-poly(A) tail, resulting in translational repression.
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