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Studies on an additional pre-beta-lipoprotein, sinking pre-beta (SPB). I. Isolation and characterization
Scandinavian Journal of Clinical and Laboratory Investigation
|January 1, 1976
Summary
Additional pre-beta-lipoprotein (LP) bands are linked to ischemic heart disease (IHD). Researchers isolated and characterized a specific fraction, identifying it as sinking pre-beta-LP (SPB), which contains the LP(a) antigen.
Area of Science:
- Cardiovascular Science
- Lipidology
- Biochemistry
Background:
- Additional pre-beta-lipoprotein (LP) bands observed during electrophoresis have been associated with ischemic heart disease (IHD).
- The precise nature and composition of these pre-beta-LP fractions require further elucidation to understand their role in IHD.
Purpose of the Study:
- To isolate and characterize a specific pre-beta-LP fraction associated with IHD.
- To determine the physicochemical properties and antigenic characteristics of this isolated fraction.
Main Methods:
- Isolation of the pre-beta-LP fraction using preparative ultracentrifugation and gel filtration.
- Characterization through density gradient analysis, lipid composition assessment, and immunological assays using specific anti-LP(a) serum.
Main Results:
- A distinct pre-beta-1-LP fraction, termed 'sinking pre-beta-LP' (SPB), was successfully isolated.
- SPB exhibits a density range of 1.050-1.080 g/ml and shares major antigenic determinants with low-density lipoproteins (LDL).
- The lipid composition of SPB closely resembles that of LDL, and the presence of the LP(a) antigen was confirmed.
Conclusions:
- The characterized sinking pre-beta-LP (SPB) is a distinct lipoprotein entity.
- SPB's association with LDL and presence of LP(a) antigen provide insights into its potential role in ischemic heart disease pathogenesis.