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Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Miniaturized dynamic light scattering instrumentation for use in microfluidic applications.

Thomas Q Chastek¹, Kathryn L Beers, Eric J Amis

¹Polymers Division, National Institute of Standards and Technology, 100 Bureau Drive Gaithersburg, Maryland 20899-8542, USA.

The Review of Scientific Instruments

|August 4, 2007

Summary

Miniaturized dynamic light scattering (DLS) instruments with microfluidics accurately measure nanoparticle sizes (10-100 nm) in small volumes. These devices enable rapid, high-throughput analysis of polymer solutions and temperature-dependent transitions.

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Published on: December 4, 2017

Area of Science:

Materials Science
Analytical Chemistry
Nanotechnology

Background:

Dynamic Light Scattering (DLS) is a crucial technique for determining particle size distribution.
Traditional DLS instruments often require larger sample volumes and can be time-consuming for high-throughput analysis.
Miniaturization and integration of microfluidics offer potential for faster, more efficient DLS measurements.

Purpose of the Study:

To develop and present five novel designs for miniaturized dynamic light scattering (DLS) instruments.
To demonstrate the capability of these instruments for accurate nanoparticle size determination in various solvents.
To showcase the utility of these instruments for high-throughput analysis of complex solution properties.

Main Methods:

Incorporation of microfluidic flow for sample handling within the DLS instrument.
Direct embedding of fiber optic probes into the microfluidic sample stream.
Integration of small stir bars for in-situ solution blending and temperature control.
Utilizing multiangle DLS measurements for enhanced data acquisition.

Main Results:

Accurate determination of particle sizes in the 10-100 nm range for samples in organic and aqueous solvents.
Successful analysis of micelle to unimer transitions in block copolymers using minimal sample mass (<20 mg).
Identification of critical micelle temperature for a triblock copolymer through integrated temperature control.
Demonstrated high-throughput capability for analyzing solution properties via systematic solvent blending.

Conclusions:

Miniaturized DLS instruments with microfluidics provide accurate and efficient particle sizing.
These instruments are well-suited for analyzing complex solution behaviors, such as polymer transitions, with minimal sample.
The developed designs offer significant advantages in speed, sample volume reduction, and analytical versatility for DLS applications.