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Updated: Jul 13, 2026

Mouse Model of Acute to Chronic Kidney Disease Transition Induced by Renal Ischemia/Reperfusion Injury
Published on: February 10, 2026
Isoflavone and the heme oxygenase system in ischemic acute kidney injury in rats
Mirian Watanabe1, Luciana Barros de Moura Neiva, Célio Xavier da Costa Santos
1Experimental Laboratory of Animal Models (LEMA) of the School of Nursing, University of Sao Paulo, Av Enéas de Carvalho Aguiar 419, São Paulo 05403000, Brazil.
Abstract:
Ischemic acute kidney injury (AKI) is mediated by reactive oxygen species and inflammatory infiltration, among others. The present study was performed to evaluate the interference of isoflavone in ischemic AKI in rats and to determine whether it was mediated by the heme oxygenase (HO) system. The treatment with dry extract of isoflavone, 8 mg/kg/day, resulted in improved renal function reducing oxidative damage. The use of HO inhibitor zinc protoporphyrin (ZnPP), 50 micromol/kg, exacerbated the rates of oxidative damage, urinary peroxides, malondialdehyde (MDA), thiols and catalyse activity, whereas an HO inducer, Hemin, 1 mg/100 g body weight, demonstrated a protective effect on renal function, with a reduction in the rates of oxidative damage. Isoflavone showed a promising role in the protection against oxidative renal dysfunction and its role is probably mediated by heme-oxygenase.
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