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Published on: January 12, 2020
CHFR gene is neither mutated nor hypermethylated in ovarian cancer
Agnieszka H Ludwig1, Mateusz Bujko, Mariusz Bidzinski
1Department of Molecular Pathology, The Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland.
Background:
CHFR is a mitotic checkpoint protein that under mitotic stress (caused also by taxanes) delays progression into mitosis.
Methods:
We looked for CHFR gene (605,209) alterations and its promoter hypermethylation in 48 ovarian carcinomas either sensitive or resistant to taxane treatment. We used single-strand conformation polymorphism analysis, sequencing and methylation-specific polymerase chain reaction.
Results:
Neither mutations in the CHFR gene nor hypermethylation of its promoter region were found. A novel single nucleotide polymorphism (Val154Val) was identified in exon 5 of alternatively spliced transcript.
Conclusions:
Our results indicate that CHFR gene status cannot serve as a molecular predictor of ovarian cancer sensitivity to taxanes.
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