Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression-linked promoter selection (ELiPS) engineers short, strong ubiquitous promoters for gene therapy applications.

bioRxiv : the preprint server for biology·2026
Same author

Characterization and immunogenicity of nanoparticle vaccines displaying embecovirus spike proteins.

Biotechnology progress·2026
Same author

Introduction.

Annual review of chemical and biomolecular engineering·2026
Same author

Optogenetic WNT signaling drives germ layer self-organization in a human gastruloid model.

bioRxiv : the preprint server for biology·2026
Same author

Evaluating the immunogenicity of clade 2 and clade 3 sarbecovirus spike protein nanoparticle vaccines in Syrian hamsters.

Vaccine·2026
Same author

Directed evolution of novel AAV capsids for enhanced delivery to mouse and human Schwann cells.

Molecular therapy. Nucleic acids·2026

Related Experiment Video

Updated: Jul 13, 2026

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
09:19

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Published on: January 4, 2015

High-throughput screening of gene function in stem cells using clonal microarrays.

Randolph S Ashton1, Joseph Peltier, Christopher A Fasano

  • 1The Howard P. Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.

Stem Cells (Dayton, Ohio)
|August 4, 2007
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microarray method for high-throughput gene function screening in stem cells. This technique enables efficient isolation and analysis of clonal neural stem cell populations for various cellular functions.

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

Related Experiment Videos

Last Updated: Jul 13, 2026

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
09:19

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Published on: January 4, 2015

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

Area of Science:

  • Stem cell biology
  • Molecular biology
  • Bioengineering

Background:

  • High-throughput screening is crucial for understanding gene function in stem cells.
  • Current methods face challenges with efficiency and applicability to difficult-to-transfect cells.
  • Developing novel platforms is essential for advancing stem cell research.

Purpose of the Study:

  • To present a microarray-based approach for high-throughput screening of gene function in stem cells.
  • To demonstrate the method's utility in growing and isolating clonal neural stem cell populations.
  • To showcase its compatibility with both gain- and loss-of-function studies.

Main Methods:

  • Utilizing soft lithographic microfabrication to create micropatterned surfaces for clonal cell seeding.
  • Constructing clonal microarrays by seeding cells at clonal density.
  • Employing parallel assays to evaluate cellular processes like proliferation, signal transduction, and differentiation.
  • Integrating cDNA libraries or RNA interference constructs for genetic manipulation.

Main Results:

  • Successfully grew and isolated clonal populations of adult and embryonic neural stem cells.
  • Demonstrated the compatibility of the microarray technique with both gain-of-function and loss-of-function studies.
  • Showcased the ability to screen large libraries in diverse mammalian cell types, including those challenging for conventional transfection.

Conclusions:

  • The developed clonal microarray approach offers a powerful tool for high-throughput gene function screening in stem cells.
  • This method facilitates the study of cellular processes and genetic manipulations in various cell types.
  • It holds significant potential for advancing stem cell research and drug discovery.