Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Issues in diisocyanate antibody testing.

M Gerald Ott1, Athena T Jolly, Amy L Burkert

  • 1BASF Corporation, Corporate Medical Department, Florham Park, New Jersey 07932, USA. gerald.ott@basf.com

Critical Reviews in Toxicology
|August 4, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In utero choline exposure alters growth, metabolism, feed efficiency, and carcass characteristics of Holstein × Angus cattle from weaning to slaughter.

Journal of animal science·2023
Same author

Dam Body Condition Score Alters Offspring Circulating Cortisol and Energy Metabolites in Holstein Calves but Did Not Affect Neonatal Leptin Surge.

Metabolites·2023
Same author

Estimates of genetic parameters for feeding behavior traits and their associations with feed efficiency in Holstein cows.

Journal of dairy science·2022
Same author

Endangered Australian top predator is frequently exposed to anticoagulant rodenticides.

The Science of the total environment·2021
Same author

High Frequency of Lead Exposure in the Population of an Endangered Australian Top Predator, the Tasmanian Wedge-Tailed Eagle (Aquila audax fleayi).

Environmental toxicology and chemistry·2020
Same author

Density trends and demographic signals uncover the long-term impact of transmissible cancer in Tasmanian devils.

The Journal of applied ecology·2018
Same journal

Microplastic trafficking in maternal-fetal system: a systematic review and quantitative profiling linking particle characteristics to developmental biology.

Critical reviews in toxicology·2026
Same journal

Addendum.

Critical reviews in toxicology·2026
Same journal

Inhalation exposure and health effects of copper particles in the context of occupational exposure limit derivation.

Critical reviews in toxicology·2026
Same journal

Screening for the ovarian toxicity of industrial chemicals - ovarian follicles and corpora lutea counts in rats, an inter- and intra-laboratory comparison.

Critical reviews in toxicology·2026
Same journal

Evaluation of the modes of action for key noncancer effects of 1,3-Butadiene: input from an independent expert panel to support derivation of data-derived extrapolation factors.

Critical reviews in toxicology·2026
Same journal

The effect of antioxidants on antineoplastic-induced male reproductive system damage: a systematic review of experimental studies.

Critical reviews in toxicology·2026
See all related articles

Diisocyanate exposure causes occupational asthma. Assays detecting IgE and IgG antibodies to diisocyanate-protein conjugates show low sensitivity for diagnosing asthma, despite high specificity for IgE. Standardization is needed for these diagnostic tools.

Area of Science:

  • Occupational toxicology
  • Immunology
  • Environmental health

Background:

  • Diisocyanates are key components in polyurethane production.
  • Occupational asthma is a significant health concern linked to diisocyanate exposure.
  • Understanding diisocyanate-induced asthma mechanisms is crucial for diagnosis and prevention.

Purpose of the Study:

  • To review literature on immunoassay detection of IgE and IgG binding to diisocyanate-protein conjugates.
  • To assess the utility of these assays as diagnostic tools and exposure indicators for diisocyanate asthma.
  • To identify limitations and suggest improvements for assay methodology and interpretation.

Main Methods:

  • Literature review of 29 studies on occupational diisocyanate exposure.

Related Experiment Videos

  • Analysis of immunoassay data for IgE and IgG antibody responses to diisocyanate-protein conjugates.
  • Evaluation of assay sensitivity, specificity, and heterogeneity across studies.

    • Main Results:

    • Significant variability in assay methodology and antibody response prevalence was observed.
    • IgE assays demonstrated low sensitivity (18-27%) but high specificity (96-98%) for confirmed diisocyanate asthma.
    • IgG detection serves as an indirect indicator of disease status and past exposure.

    Conclusions:

    • Current immunoassay utility is limited by lack of standardization and population norms.
    • Assays do not consistently improve disease prediction or exposure confirmation over other indicators.
    • Improvements in test performance and interpretability require method standardization and validation.