[Cloning and bioinformatic analysis of the cDNA encoding a novel heat-shock factor OsHSF13 in Oryza sative L]

As a crucial transcription factor family,heat-shock factors were mainly analyzed and characterized in tomato and Arabidposis. In this study, we isolated a putative heat-shock factor OsHSF13 that interacted specifically with heat-shock element (HSE) from Oryza sativa L. by yeast one-hybrid method. The full-length cDNA of OsHSF13 has a 1047bp open reading frame (ORF). It has HSF special DNA-binding domain (DBD) and oligomerization domain (OD). Analysis of the deduced amino acid sequences revealed that OsHSF13 was a class C heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor. Bioinformatic analysis showed that the sequence and structure of OsHSF13's DNA binding domain had a high similarity with LpHSF24.