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[Effects of EGb 761 on the cell apoptosis induced by H2O2 in RIN-m beta cells]
Chun-ling Ye1, Yong-liang Jin, Kai-he Ye
1Department of Pharmacology, Pharmacy College of Jinan University, Guangzhou 510632, China. yechunling2005@163.com
Objective:
To investigate the effects of ginkgo biloba extract (EGb 761) on apoptosis induced by hydrogen peroxide (H2O2) in RIN-m beta-cells.
Methods:
The apoptotic model was made by H2O2 exposed for six hours with a concentration of 500 micromol/L The cytotoxicity was measured by MTT. Hoechst 33258 fluorescent staining were used to detect the protective effect of EGb 761 on the apoptosis of RIN-m beta-cells induced by H2O2. Annexin V-PI double staining of Flow cytometry were used to detect apoptosis quantitively.
Results:
Compare to control group, after exposed to 500 micromol/L H2O2 for 6 hours, the apoptosis rate incereased and cell survival rate were decreased considerably (P < 0.01). Pretreated for 10 hours with EGb 761, the flow cytometry results showed that the apoptosis rate decreased and cell survival rate were increased considerably (P < 0.01, compared to H2O2 control group).
Conclusion:
EGb 761 can decrease RIN-m beta-cells damage and apoptosis induced by H2O2.