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A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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[Protective effects of beta-asarone on cultured rat cortical neurons damage induced by glutamate]
Yi-zhi Chen1, Qi-wen Wang, Yi Liang
1The First Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510405, China.
Objective:
To study the protective effects of beta-asarone on cultured rat cortical neurons damage induced by glutamate.
Methods:
The protective effects of beta-asarone on cultured rat cortical neurons after glutamate intoxication were observed with morphology, extent of damage, livability, Intracellular calcium concentration and apoptosis ratio.
Results:
Morphological changes, LDH leakage and intracellular calcium concentration increasing, cell survival decreasing were observed in cultured rat cortical neurons exposured to glutamate; 7.5, 15, 30 microg/ml beta-asarone could increase cell survival, decrease LDH leakage and apoptosis ratio; 15, 30 microg/ml beta-asarone could reduce intracellular calcium concentration.
Conclusion:
The results suggest that beta-asarone prevents the toxicity of glutamate, and it maybe attribute to its effect of anticalcium.
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