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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 14, 2026

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

A simple array platform for microRNA analysis and its application in mouse tissues.

Xiaoqing Tang1, Jozsef Gal, Xun Zhuang

  • 1Gene Suppression Laboratory, Department of Plant and Soil Sciences and Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, Kentucky 40546-0236, USA.

RNA (New York, N.Y.)
|August 7, 2007
PubMed
Summary
This summary is machine-generated.

A new array platform detects 553 human and mouse microRNAs (miRNAs), crucial for gene regulation. This tool identifies tissue-specific and ubiquitous miRNAs, aiding diverse biological research.

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression post-transcriptionally.
  • miRNA conservation between humans and mice enables cross-species expression analysis.
  • Understanding miRNA expression is vital for numerous biological processes.

Purpose of the Study:

  • To develop and validate a simple array platform for detecting 553 human and mouse miRNAs.
  • To investigate potential cross-reactions between mature miRNAs and their precursors.
  • To analyze miRNA expression in mouse central nervous system and other organs.

Main Methods:

  • Design and optimization of probes for miRNA detection.
  • Hybridization parameter optimization for enhanced specificity.
  • Array analysis of miRNA expression in mouse CNS, liver, and heart tissues.
  • Validation using Northern blot analysis and comparison with existing data.

Main Results:

  • Detection of 553 nonredundant miRNAs, covering the entire set for humans and mice.
  • Identification of differentially expressed organ/tissue-associated miRNAs and ubiquitously expressed miRNAs.
  • Discovery of new tissue-associated miRNAs, such as liver-associated miR-194.
  • Limited cross-reaction between mature miRNAs and precursors, with specific exceptions (miR690, miR709, miR720).

Conclusions:

  • The developed array platform is reliable and effective for detecting a comprehensive set of miRNAs.
  • The platform successfully identified known and novel tissue-specific miRNAs.
  • A new data normalization method using Northern blot analysis was introduced.
  • This miRNA array platform is a valuable tool for broad biological studies.