An undifferentiated sarcoma in a rat resembling extraskeletal myxoid chondrosarcoma in man

A soft tissue tumor occurring in the inguinal subcutaneous tissue was detected in a 109-week-old male F344 rat. Macroscopically, the tumor mass showed no skeletal relationship and a gelatinous multinodular appearance. Histologically, the tumor consisted of irregular lobules separated by scant fibrous septa. In each lobule, tumor cells were arranged in cords and strands in the plentiful myxoid stroma. The tumor cells showed marked pleomorphism, and had large, round to ovoid nuclei and abundant eosinophilic cytoplasm. Mitotic figures were often seen. Myxoid stroma of the tumor contained a large amount of acid mucopolysaccharides and collagen type II, which were demonstrated by histochemistry and immunohistochemistry respectively. The tumor cells showed positive immunoreactivities for vimentin and S100 protein. Ultrastructural examination revealed that the tumor cells had a large amount of mitochondria, Golgi complex and dilated rough endoplasmic reticulum containing amorphous material, and the myxoid stroma contained collagenous fibrils and proteoglycan particles. Based on these results, the present tumor in a rat resembled extraskeletal myxoid chondrosarcoma in man.