High spatial resolution multisite EPR oximetry of transient focal cerebral ischemia in the rat

In vivo electron paramagnetic resonance (EPR) spectroscopy can provide direct noninvasive, continuous, and repeatable measurements of oxygen in tissues. High-spatial-resolution multisite (HSRMS) oximetry is an EPR technique that uses applied magnetic field gradients to extend this capability to multiple implanted probes within the sample and accurately to estimate their respective local pO(2) values. These capabilities are crucial in experiments in which pO(2) varies across space and time and in which information about these variations is needed to describe physiologic and pathophysiologic phenomena and evaluate their responses to interventions such as therapy. One important application is the investigation of transient focal ischemia in the rat brain and the effects of treatment with hyperoxygenation. We used HSRMS oximetry with overmodulation to measure brain tissue oxygenation in a rat stroke model using lithium phthalocyanine as the oxygen probe. Oxygen measurements were made in a small cohort of rats at four implant sites during ischemia and reperfusion after transient focal ischemia initiated by occlusion of the middle cerebral artery. These measurements demonstrate the capabilities of the HSRMS oximetry technique and set the stage for more extensive physiologic studies.