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Updated: Jul 13, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Published on: December 27, 2010
The heterosexual HIV type 1 epidemic in Papua New Guinea is dominated by subtype C
Claire E Ryan1, Janet Gare, Suzanne M Crowe
1The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia, 3004. claire.ryan@burnet.edu.au
Abstract:
Papua New Guinea is in the midst of a generalized HIV epidemic. As part of a larger behavioral survey aiming to further characterize the HIV epidemic occurring in PNG, samples were collected from 1175 participants from seven different provinces. Seventy-one (6%) of these samples were HIV-1 positive, and 35 (49%) successfully underwent a double nested RT-PCR that was designed to amplify the C2-V4 region of the HIV-1 envelope. Sequence analysis showed that 33 (94%) samples were subtype C and the remaining 2 (6%) were subtype B. Further phylogenetic analysis demonstrated that there was no province-specific clustering among the samples and that within the global pandemic, PNG subtype C isolates most closely resembled those from East Africa.
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