Related Experiment Video
Updated: Jul 13, 2026

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
Published on: December 12, 2015
Comparison of bicarbonate and base excess values analyzed by four different blood gas analyzers
Katsuhito Nakamaru1, Noboru Hatakeyama, Masana Yamada
1Department of Surgical Operation, Toyama University Hospital, 2630 Sugitani, Toyama 930-0194, Japan.
Abstract:
Comparison of the calculation formula, reproducibility, correlation and variation of bicarbonate ion concentration (HCO(3) (-)), and base excess value (BE) among four blood-gas analyzers was performed. No HCO(3) (-) and BE values calculated from the formulas showed any clinically significant difference, and all analyzers showed good correlation on their measurements. On the actual measurement of a specific sample, however, BE values from the same sample ranged between -6.3 and -15.7, which might affect therapeutic strategy. Caution should be taken for the assessment of data if different types of blood-gas analysis devices are used in the same facility.
Related Concept Videos
Diagnosing Acidosis and Alkalosis
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2 and HCO3− values are examined to...
Titration of Polyprotic Base with a Strong Acid
Blood Studies I: ABG and VBG
Arterial Blood Gas (ABG)
Arterial Blood Gas (ABG) studies are crucial for assessing the lungs' ability to supply oxygen and remove carbon dioxide, reflecting the patient's ventilation status. They also help understand the kidneys' capacity to reabsorb or...
Bicarbonate-Carbonic Acid Buffer
Disorders of Acid-Base Balance
Respiratory Acidosis and Alkalosis
Respiratory acidosis occurs due to an increase in the partial pressure of carbon dioxide PCO2 in the blood. It often arises from shallow breathing or impaired gas exchange caused by...
Titration of a Weak Acid with a Strong Base
