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Updated: Jul 13, 2026
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
Published on: December 10, 2012
[Rapid detection of chromosomes X and Y using dual-color primed in situ labeling technique]
Jian-bin Yang1, Zheng-yan Zhao
1Children's Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003 P. R. China.
Objective:
To develop a rapid method for detection of chromosomes, and to present a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes using modified dual-color primed in situ labeling (PRINS) technique.
Methods:
Chromosomes X and Y were detected and analyzed by the modified dual-color PRINS in 205 amniotic fluid samples.
Results:
The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 98% of the samples PRINS reactions with primers X and Y were successfully induced. One sample was properly identified and correctly scored as 47, XXY.
Conclusion:
The results suggest that PRINS technique is rapid, simple and cost-effective. It is also sensitive and specific, and thus useful for rapid detection of chromosome abnormalities.
