Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process.

Lars Aumann1, Massimo Morbidelli

  • 1ETH Zurich, Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Hoenggerberg, HCI, 8093 Zurich, Switzerland.

Biotechnology and Bioengineering
|August 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026
Same author

On the Prediction of pDNA Productivity Across Diverse Bioprocesses Using Ensemble Hybrid Models.

Biotechnology and bioengineering·2026
Same author

High-density perfusion cultures of the marine bacterium Rhodovulum sulfidophilum for the biomanufacturing of oligonucleotides.

Journal of biotechnology·2024
Same author

UV-based dynamic control improves the robustness of multicolumn countercurrent solvent gradient purification of oligonucleotides.

Biotechnology journal·2024
Same author

Soft sensor based on Raman spectroscopy for the in-line monitoring of metabolites and polymer quality in the biomanufacturing of polyhydroxyalkanoates.

Journal of biotechnology·2023
Same author

Role of the gradient slope during the product internal recycling for the multicolumn countercurrent solvent gradient purification of PEGylated proteins.

Journal of chromatography. A·2023
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

A new semicontinuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process using only three columns offers improved performance over the original six-column setup. This optimized chromatographic technique effectively purifies complex peptide mixtures, including Calcitonin.

Area of Science:

  • Chromatographic techniques
  • Process engineering
  • Biopharmaceutical purification

Background:

  • The continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process is a recent advancement in chromatography.
  • Scaling down complex chromatographic processes presents significant engineering challenges.
  • Optimizing purification efficiency is critical for industrial peptide production.

Purpose of the Study:

  • To develop and validate a semicontinuous, reduced-column MCSGP setup.
  • To compare the performance of a 3-column MCSGP unit with the original 6-column process.
  • To assess the predictive capability of a simulation model for process design.

Main Methods:

  • Implementation of a 3-column MCSGP system with three gradient pump modules.

Related Experiment Videos

  • Experimental purification of an industrial peptide mixture containing Calcitonin (46%) using reversed-phase chromatography.
  • Comparison of experimental results with data generated from a process simulation model.

    • Main Results:

    • The 3-column MCSGP unit achieved performance equivalent, and in some aspects superior, to the 6-column process.
    • An additional degree of freedom, variable switching times, enhanced the 3-column system's performance.
    • The simulation model accurately predicted the system behavior under defined operating conditions.

    Conclusions:

    • The semicontinuous 3-column MCSGP process is a viable and efficient alternative to continuous setups.
    • The developed simulation model is suitable for predicting system behavior and optimizing operating conditions for MCSGP.
    • This optimized process facilitates the efficient purification of complex peptide mixtures in industrial settings.