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Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
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Published on: July 6, 2021

Advancing mammalian cell culture engineering using genome-scale technologies.

Timothy J Griffin1, Gargi Seth, Hongwei Xie

  • 1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA. tgriffin@umn.edu

Trends in Biotechnology
|August 8, 2007
PubMed
Summary

Optimizing mammalian cell lines for protein therapeutic production requires understanding complex system-wide properties. Genome-scale technologies offer powerful tools for engineering more efficient cell lines for healthcare advancements.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Bioprocessing

Background:

  • Mammalian cell-derived protein therapeutics have revolutionized healthcare over the past 20 years.
  • There is a continuous need for more cost-effective and efficient cell lines for high-quality protein production.

Purpose of the Study:

  • To review current knowledge on cell properties crucial for designing efficient protein-producing mammalian cell lines.
  • To highlight the application of genome-scale technologies in cell culture engineering.
  • To discuss emerging technologies with potential for advancing protein therapeutic production.

Main Methods:

  • Review of existing literature on mammalian cell line engineering for protein production.
  • Analysis of studies utilizing genomics, transcriptomics, and proteomics in cell culture.
  • Exploration of alternative and emerging technologies for bioprocessing.

Main Results:

  • Optimal producer cell line characteristics are complex and system-wide, not attributable to single genes.
  • Genome-scale technologies are increasingly relevant but still underutilized in cell culture engineering.
  • Several alternative and emerging technologies show promise for enhancing protein therapeutic manufacturing.

Conclusions:

  • A holistic, system-wide understanding is essential for engineering superior mammalian producer cell lines.
  • Further integration of genome-scale and novel technologies can significantly improve protein therapeutic production efficiency and quality.
  • Advancements in cell line engineering are critical for the future of biopharmaceutical development.