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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
Published on: January 7, 2019
Selection and optimization procedure of synbiotic for cholesterol removal
Fang Zhang1, Xiaomin Hang, Xiaobing Fan
1School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, PR China.
Anaerobe
|August 8, 2007
Summary
Researchers optimized a synbiotic combination of probiotics and prebiotics for cholesterol removal. They selected Lactobacillus plantarum LS12 and specific prebiotics, enhancing bile tolerance and growth for effective cholesterol reduction.
Area of Science:
- Microbiology and Gut Health
- Food Science and Technology
Background:
- Synbiotics, combining probiotics and prebiotics, show potential for health benefits, including cholesterol management.
- Optimizing synbiotic formulations requires understanding probiotic-prebiotic interactions, fermentability, and strain-specific responses.
Purpose of the Study:
- To establish a selection and optimization procedure for a synbiotic combination to enhance in vitro cholesterol removal.
- To identify suitable probiotic strains and prebiotic compounds that support each other for improved functionality.
Main Methods:
- Evaluated prebiotic fermentability by various probiotic strains, including Lactobacillus plantarum and Lactobacillus ruminis.
- Assessed strain-specific prebiotic effects on bile tolerance and growth.
- Utilized response surface methodology to optimize the synbiotic combination for cholesterol removal.
Main Results:
- Lactobacillus plantarum LS12 demonstrated superior prebiotic fermentation and bile tolerance, making it a prime candidate for synbiotic development.
- Five prebiotics (FOS, stachyose, GOS, IMO, and mannitol) were selected for their ability to enhance LS12's bile tolerance and growth.
- Mannitol and GOS were identified as significant factors in optimizing cholesterol removal via the synbiotic combination.
Conclusions:
- A robust procedure for selecting and optimizing synbiotics was established.
- The optimized synbiotic formulation, featuring L. plantarum LS12 and specific prebiotics, shows promise for effective in vitro cholesterol removal.
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